“Molecular scissors” is
(a) Helicase
(b) DNA ligase
(c) DNA polymerase
(d) Restriction endonuclease
With the help of DNA ligase, a donor DNA fragment is joined. It is called :
(a) Molecular cloning
(b) Tissue culture
(c) Protoplasmic fusion
(d) Splicing
The organism which is used for gene transfer in higher organisms is
(a) Agrobacterium tumefacians
(b) E. coli
(c) Acetobacter
(d) Bacillus thuringenesis
The first artificial gene was synthesized by
(a) Nirenberg
(b) Mendel
(c) Morgan
(d) Khorana
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Restriction enzyme EcoR I cleavages DNA at the sequence :
(a) AAGCTT
(b) AAGTTC
(c) GTATATC
(d) GAATTC
Which one of the following is regarded as a natural genetic engineer?
(a) Klebsiella frosteri
(b) Bacillus subtites
(c) Agrobacterium tumefaciens
(d) Neurospora
Plants are genetically engineered with novel genes by
(a) Protoplast fusion
(b) Recombinant DNA technology
(c) Embryo rescue technique
(d) Recombination breeding
Advancement in genetic engineering has been possible due to the discovery of :
(a) Transposons
(b) Endonucleases
(c) Exonucleases
(d) Oncogenes
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Reproducing new plants by cells instead of seeds is known as
(a) Biofertilizer
(b) Tissue culture
(c) Mutation
(d) Antibiotics
In which bacteria gene of insulin was cloned
(a) E. coli
(b) Clostridium
(c) Shigella
(d) Diplococcus
A plasmid :
(a) Lives together with chromosomes
(b) Shows dependent assortment
(c) Can replicate independently
(d) Cannot replicate
What does Bt stand for in the popular crop Bt-cotton?
(a) Biotechnology
(b) Bacillus tomentosa
(c) Best
(d) Bacillus thuringenesis
Modified antibiotics are manufactured by the technique of
(a) Ultrafiltration
(b) Ultracentrifuge
(c) Vernalization
(d) Genetic engineering
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Which one is used as a vector?
(a) Cyanophage
(b) Bacteriophage
(c) Plasmid
DNA finger printing is useful for the study of :
(a) Forensics
(b) Polymorphism
(c) Identity and relationships
(d) All the above
The first gene therapy used cells altered outside the recipient’s body and is called
(a) in situ
(b) in vivo
(c) ex vivo
(d) Vaccine
Widely used tool in genetic engineering of crop plants involves
(a) protoplast fusion
(b) Microinjection
(c) Agrobacterium mediation
(d) Transposon mediation
Cloning means to:
(a) Replace original genotype
(b) Preserve genotype
(c) Production of HGH gene in Escherichia coli
Which one of the following is indispensable in genetic engineering?
(a) DNA ligase
(b) DNA polymerase
(c) RNA polymerase
(d) Reverse transcription
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Restriction endonucleases are:
(a) Used in genetic engineering for uniting two DNA molecules
(b) Used for in vitro DNA synthesis
(c) Present in mammalian cells for degeneration of DNA of dead cells
(d) Synthesised by bacteria for their defence
Insect resistance transgenic cotton has been produced by inserting a piece of DNA from
(a) An insect
(b) A wild relative of cotton
(c) A bacterium
(d) A virus
Genetic drift operates only in
(a) Smaller population
(b) Larger population
(c) Mendelian population
(d) Island population
Which of the following is important in genetic engineering?
(a) DNA polymerase
(b) Restriction endonuclease
(c) RNA polymerase
(d) Nuclease
Chemical knives/molecular scissors of DNA are:
(a) Endonucleases
(b) Polymerases
(c) Ligases
(d) Transcriptases
Two bacteria found to be very useful in genetic engineering experiments are
(a) Nitrosomonas and Klebsiella
(b) Escherichia and Agrobacterium
(c) Nitrobacter and Azotobacter
(d) Rhizobium and Diplococcus
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Abnormal genes are replaced by normal genes through
(a) Gene therapy
(b) Medicines
(c) Cloning
(d) Radiation
Plasmids are vectors for gene cloning because they:
(a) Self-replicate in bacterial cells
(b) Replicate freely outside bacterial cells
(c) Can be multiplied in culture
(d) Can be multiplied in laboratories using enzymes
Which one of the following is called the resistance transfer factor?
(a) F–factor
(b) R–factor
(c) Col–factor
HIV has a protein coat and a genetic material which is
(a) ss DNA
(b) ss RNA
(c) ds DNA
(d) ds RNA
Bacterial plasmid contains:
(a) RNA
(b) RNA + protein
(c) DNA
(d) Photosynthetic structures
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Which enzyme is used for the separation of genetic material?
(a) Ligase
(b) Restriction endonuclease
(c) Hydrolase
(d) Amylase
Which one of the following is a first genetic engineered medicine?
(a) Humulin
(b) Somatostatin
(c) Interferon
(d) Human growth hormone
Transgenic plants are developed by :
(a) Introducing foreign genes
(b) Introducing gene mutations
(c) Deleting certain chromosome parts
(d) Stopping spindle formation
A human gene product can be produced by genetically engineered bacteria. This is possible because the
(a) Human chromosome can replicate in bacterial cells
(b) The mechanism of gene regulation is identical in humans and bacteria
(c) The genetic code is universal
(d) Bacterial cells can carry out the splicing reaction
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In transgenics, expression of transgene in target tissue is determined by
(a) Promoter
(b) Reporter
(c) Enhancer
(d) Transgene
It is now possible to breed plants and animals of desired characters through :
(a) Tissue culture
(b) Genetic engineering
(c) Ikebana technique
(d) Chromosome engineering
Which of the following organelles is related with Genetic Enginering?
(a) Golgi apparatus
(b) Lysosomes
(c) Mitochondria
(d) Plasmids
Genetically engineered human insulin is manufactured by the use of
(a) Pseudomonas
(b) Ashbya gossypii
(c) Rhizopus
(d) Escherichia coli
Restriction endonuclease is employed for cutting:
(a) A single stranded DNA
(b) Double stranded DNA
(c) RNA fragment
(d) mRNA
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Somatic hybrids are produced by
(a) Protoplast fusion
(b) Tissue culture
(c) Pollen culture
(d) Hybridoma process
Genetic engineering is connected with
(a) Eugenics
(b) Euthencis
(c) Euphenics
(d) All of these
Introduction of foreign genes for improving genotype is
(a) Biotechnology
(b) Tissue culture
(c) Vernalization
(d) Genetic engineering
Which of the following genetic diseases is not caused due to autosomal genemulaiton?
(a) Albinism
(b) Alcaptonuria
(c) Haemophilia
(d) Phenylketonuria
The term ‘Genetics’ was introduced by
(a) William Harvey
(b) Johannsen
(c) William Bateson
(d) Hugo de Vries
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The first great geneticist was
(a) Miller
(b) Mendel
(c) Galton
(d) Morgan
Which of the following is related to genetic engineering?
(a) Plastid
(b) Plasmid
(c) Heterosis
(d) Mutation
Name the scientist who was awarded the Nobel Prize for his genetic studies on the linear arrangement of genes on the chromosomes in the fruitfly, Drosophila melanogaster
(a) C.F. Wolff
(b) T.A. Knight
(c) J. Swammerdam
(d) T. H. Morgan
Transgenic plants are
(a) Plants have no genes
(b) Plants in which genes are present in an opposite or transposition
(c) Plants in which genes have no function to perform
(d) Plants in which genes of another organism have been implanted
Which genetic disorder is caused due to autosomal numerical change?
(a) Hypertrichosis
(b) Edward syndrome
(c) Haemophilia
(d) Albinism
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Which genetic disorder develops due to chromosomal structural change?
(a) Cri – du – chat syndrome
(b) Super female
(c) Super male
(d) All the above
Cistron concept of the gene is that
(a) The gene is a unit of function
(b) Gene is a unit of recombination
(c) Gene is a unit of mutation
(d) None of these
The genotypes of a dominent parent is determind by crossing it with the hybrid. This cross is known as
(a) Red cross
(b) Long cross
(c) Back cross
(d) Test cross
